Making custom diets is our business. Every diet we make is custom. Our staff of scientists work daily with researchers from around the world to develop unique formulations for individual studies. Research Diets, Inc. has created over 25,000 "Original" formulas and is able to produce and ship custom diets in 5 to 7 business days.
Because of the large number of formulas in our database, not all can be found on the website. If you don't find what you're looking for, please contact us. We save all our formulations and can report, repeat and revise as needed.
Add Test Compounds
RDI Test Compounds
Simple, Safe Dosing
Research Diets will incorporate your compounds into any experimental diet and ship within 5 to 7 days. Feeding test compounds eliminates dosing related stress to the animal, eliminates vehicle effects, and saves time and labor.
Repeat Formula and Dose Response
A consistent purified ingredient OpenSource diet formulation provides a clean, repeatable control diet for your research. Precise, graded addition of test compounds to your specified control diet allows evaluation of dose-response effects in your animal model. We can blend your compound homogeneously into any diet, down to ppm and even as low as parts-per-billion.
Pelleted Micro Batches
Research Diets will incorporate test compounds into pelleted micro batches as small as 500 grams for those compounds that are in limited supply. Our production facility is well equipped to pellet small batches at precise doses.
STEP 1. Complete a Material Transfer Information Form (MTIF) prior to sending us any test compounds. This is a simple procedure designed to assure that we handle your compounds efficiently, effectively, and safely.
STEP 2. Send the MSDS for your compound.
STEP 3. Calculate the diet dose of your compound. Use the calculator below.
|Fill in Variables||Units|
|Single Daily Dose||SD=||mg Compound/kg BW/day|
|Body Weight||BW=||gm BW/animal|
|Daily Food Intake||FI=||gm Diet/day|
|Diet Dose||DD=||mg Compound/kg Diet|
We can make as little as 0.5 kg of diet with added compounds. Generally, we will require a small increment of additional compound to provide for losses during processing. We will routinely return to you the original container containing excess compound if any, or empty, as the case may be.
When sending us compounds from outside the U.S. please contact Dima Paone for direction on what paperwork may be required.
The growing use of zebrafish as models of human development and disease requires some standardization in the field. In 2018, the NIH held a workshop entitled “Defined Reference Diets for Zebrafish and Other Aquatic Biomedical Research Models: Needs and Challenges”. One of the take-home messages from this workshop was the need to use defined diets so that the zebrafish research community would be able to easily control and modify the diet, repeat studies and compare data across laboratories.
To help in that effort, we have, with valuable input from Dr. Stephen Watts from UAB, formulated and produced a purified ingredient zebrafish diet called Z10001.19D. At the CRO, ZeClinics, the diet was fed to zebrafish at 21 days post fertilization for 16 weeks and compared favorably with another fishmeal-based diet. We feel this diet is a good starting point for a ‘base’ diet for zebrafish while at the same time understanding that, as in any field, as new data emerges it may be necessary to modify the formula in order to expand its usefulness.
Such data can come from the zebrafish research community. We invite you to evaluate this diet (and modifications of it) under different conditions while studying your phenotypes of choice. We look forward to discussing how using a purified ingredient diet could help in your next zebrafish study.
Diet Considerations for Germ Free Studies
Germ Free Studies
The human gut is host to trillions of microorganisms and is unique to individuals. Numerous environmental factors are known to influence the microbiome, including short and long term dietary trends. It is now understood that the gut microbiome influences host health and is involved in disease onset. As such, the volume of research revolving around the gut-microbiome’s involvement in health and disease has escalated in the past two decades. Mouse models are the most commonly used mammalian species in mechanistic studies of health and disease. To fully understand the role of the gut microbiome in disease states, it is necessary to perform research studies in a variety of mouse models including germ-free or gnotobiotic colonies. Germ free animals are maintained so that their gut microbiomes are uncolonized i.e. they are not hosts to any other living organisms. Similarly, gnotobiotic animals are free of pathogens, but may be host to specific organisms in a controlled environment.
Vitamin and Mineral Deficient Diets
Vitamin & Mineral Deficient Diets
Animal models have been, and continue to be crucial in understanding the etiology of metabolic diseases in humans. Nutritional science research during the 20th century has shown that diet is a powerful environmental tool capable of changing the phenotype of an animal, and thus improve our understanding of different diet-related metabolic diseases. Examples include obesity, diabetes, dyslipidemia, non-alcoholic steatohepatitis, atherosclerosis, and hypertension. To study these disorders in animals, researchers typically use diets that contain excess levels of fat and sucrose, among other modifications. Animal diets can also be modified to create vitamin and mineral deficiencies which allows researchers to define the requirements of these nutrients.