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Diet and Imaging

In order to produce high quality in vivo images using fluorescence imaging technologies, it is important to have as low a background signal as possible. It has been shown that laboratory animal diets containing chlorophyll fluoresce at 680 nm, which can interfere with the imaging of many common in vivo fluorophores such as GFP or Alexafluor 650 and 680. The confounding fluorescent signal they produce as they pass through the gastrointestinal tract makes quantification of true signal difficult. It appears that unrefined chlorophyll-containing ingredients, particularly alfalfa found in grain-based chow diets, are responsible for this ‘noise’. Using a purified diet eliminates chlorophyll and provides the clean background in which to view your images. Consult with one of our scientists on a formula for your specific study.

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Sara 1045

Sara Sturgess, M.S., R.D.N.

Project Manager and Scientist

Laura Griffin, Ph.D.

Project Manager and Scientist
Mike 0923

Michael A. Pellizzon, Ph.D.

Senior Scientist