A number of custom OpenSource diet formulations have been widely published and have become the standards for many diet-induced disease models. These custom diets are kept in-stock in pelleted form and ready for shipment. Our in-stock custom diets provide a clean, open formula background in which to make modifications. See our custom diets page for more information on other custom formulations.

The following is a list of our in-stock purified diets:

Diet Formula Description Google Scholar
A06071302 L-Amino Acid Diet With 60 kcal% Fat With 0.1% Methionine and No Added Choline References
A02082002BR Methionine and Choline Deficient  L-Amino Acid Diet References
D09100310 Rodent Diet With 40 kcal% Fat (Mostly Palm Oil), 20 kcal% Fructose and 2% Cholesterol References
D11112201 OpenStandard Diet With 15 Kcal% Fat References
D17010103 Rodent Diet With 40 kcal% Fat With Palmitic Acid and Trans Fat Levels Similar to D09100301 References
D10001 AIN-76A Rodent Diet References
D10012G AIN-93G Growing Rodent Diet References
D10012M AIN-93M Mature Rodent Diet References
D12079B RD Western Diet References
D12108C Clinton/Cybulsky High Fat Rodent Diet With Regular Casein and 1.25% Added Cholesterol References
D12266B Purified Rodent Diet to Match Condensed Milk Diet (MHF Diet, 32.5 kcal% Fat) References
D12331 Rodent Diet With 58 kcal% Fat and Sucrose References
D12450B Rodent Diet With 10 kcal% Fat References
D12450H Rodent Diet With 10 kcal% Fat (Matching Sucrose to D12451) References
D12450J Rodent Diet With 10 kcal% Fat (Matching Sucrose to D12492) References
D12450K Rodent Diet With 10 kcal% Fat (No Sucrose) References
D12451 Rodent Diet With 45 kcal% Fat References
D12492 Rodent Diet With 60 kcal% Fat References

All our in-stock diets can be irradiated. To order irradiated diets add the letter "i" to the end of the diet number. For example D12492i.

Diet-Induced Obesity (DIO)

Research Diets, Inc. is well known as the gold standard in high-fat diets for diet-induced obesity research worldwide. Below are the most widely published diets for these studies. All high-fat diets are not the same. We have made hundreds of modifications to these formulas to meet individual researcher needs. Please consult with one of our scientists before choosing a high-fat diet for your next study. Read more.

The following is a list of our in-stock DIO Series Diets:

Diet Formula Description Google Scholar
D12492 DIO (VHFD) 60 kcal% fat References
D12451 DIO (HFD) 45 kcal% fat References
D12450B Control (LFD) 10 kcal% fat 35% Sucrose References
D12450H Control (LFD) 10 kcal% fat D12451 Match 17% Sucrose References
D12450J Control (LFD) 10 kcal% fat D12492 Match 7% Sucrose References
D12450K Control (LFD) 10 kcal% fat No Sucrose References

NASH Diets

Methionine and Choline Deficient (MCD) Diets
Among the different approaches for diet-induced NAFLD in rodents, MCD diets produce the most severe NASH phenotype in the shortest timeframe. MCD diets are formulated with the replacement of whole protein (such as casein) in purified diets with crystalline amino acids and the removal of both methionine and choline. Read more

Choline Deficient (CD) Diets
Typically, CD diets used in fatty liver disease studies tend to contain higher levels of fat (45-60 kcal%) and these diets can induce steatosis, inflammation and fibrosis without the reduction in body weight typically found when feeding MCD diets, making CD diets more appealing to some researchers. Read more

High-Fat, High-Fructose, High-Cholesterol Diets
Studies in C57BL/6 mice fed a HFD or high-fat, high-fructose (HFHFr) diet (58 Kcal% fat) with high-fructose corn syrup in water for 16 weeks showed that fructose consumption was necessary for the progression from liver fat deposition to fibrogenesis. Read more

The following is a list of our in-stock NASH Diets:

Diet Formula Description Google Scholar
A06071302 L-Amino Acid Diet With 60 kcal% Fat With 0.1% Methionine and No Added Choline References
A02082002BR Methionine and Choline Deficient  L-Amino Acid Diet References
D09100310 Rodent Diet With 40 kcal% Fat (Mostly Palm Oil), 20 kcal% Fructose and 2% Cholesterol References
D17010103 Rodent Diet With 40 kcal% Fat With Palmitic Acid and Trans Fat Levels Similar to D09100301 References

Control Diets

Report, Repeat and Revise
When choosing a control diet, one should ask three questions: Can I report it (can I tell others exactly what my animals were fed)? Can I repeat it (is there diet variability and will I be able to get the same results next year)? Can I revise it (as my hypotheses change, can I easily change the dietary components while keeping it otherwise matched to previous diets)? The answer should be “yes” to all three. Additionally the control diet formula should be matched to that of the experimental diet.

Pellizzon and Ricci , The common use of improper control diets in diet-induced metabolic disease research confounds data interpretation: the fiber factor. Nutrition & Metabolism (2018) 15:3. Download publication

The following is a list of our in-stock Control Diets:

Diet Formula Description Google Scholar
D11112201 OpenStandard Diet With 15 Kcal% Fat References
D12450B Control (LFD) 10 kcal% fat 35% Sucrose References
D12450H Control (LFD) 10 kcal% fat D12451 Match 17% Sucrose References
D12450J Control (LFD) 10 kcal% fat D12492 Match 7% Sucrose References
D12450K Control (LFD) 10 kcal% fat No Sucrose References

Ready to speak with a scientist?

Vijay 6572

Vijay Indukuri, Ph.D.

Project Manager and Scientist
Sara 1045

Sara Sturgess, M.S., R.D.N.

Project Manager and Scientist
Holly 0947

Jia-Yu (Holly) Ke, Ph.D.

Project Manager and Scientist